# § V. Application to Colloidal Dispersions

Because the positions of microscopic particles, such as atoms, are hard to measure, experimental applications of the configurational temperature have been relatively limited (5). Colloidal dispersions differ from many experimental systems in that their constituents' motions can be tracked quite easily. Unlike most macroscopic model systems, they can be thoroughly equilibrated through the particles' intimate contact with the suspending fluid. Furthermore, an effective potential between two particles often can be defined. For this reason, colloidal dispersions offer an almost ideal experimental test-bed for studying the configurational temperature and related ideas. These ideas, in turn, provide valuable new tools for assessing the microscopic state of this important state of matter.

This section describes methods for estimating
the configurational and hyperconfigurational
temperatures of colloidal dispersions from digital video microscopy
measurements of their microscopic dynamics.
Emphasis is placed on how to account for inevitable experimental
errors in systems of limited size whose interactions may not be
fully characterized *a priori*.
In particular, we analyze the structure and dynamics of dilute
monolayers of charge-stablized
colloidal spheres confined by planar surfaces, on the one hand using convergence of
different temperature expressions as a thermodynamic self-consistency test for
independently measured effective pair potentials,
and on the other as a new approach to measuring the pair potential directly.

## § V.1. Interactions in charge-stabilized colloid

Considerable attention has been focused in recent years on colloidal interactions, particularly in light of experimental observations that challenge theoretical predictions. For example, charged colloidal spheres of diameter and charge number dispersed in an electrolyte interact with each other directly through their Coulomb repulsion and also indirectly through their influence on the surrounding distribution of simple, atomic-scale ions. Poisson-Boltzmann mean-field theory predicts an overall screened-Coulomb repulsion (8) of the form

(23) |

where is the thermal energy scale at absolute temperature ,
is the spheres' center-to-center separation,
and is the Bjerrum length
in a medium of dielectric constant .
If the electrolyte has a total concentration of monovalent ions,
then the Debye-Hückel screening length,
,
sets the range of the effective electrostatic interaction in the mean-field
approximation.
This is an effective interaction because it results from an
average over the simple ions' degrees of freedom.
When viewed in this light, it is not surprising that measurements might
differ from predictions based on Eq. (23).
More surprising is that like-charged colloidal spheres appear to
*attract*
each other under some circumstances, in qualitative disagreement with
Eq. (23).
One of the goals of the present study is to apply the configurational temperature
formalism to resolve some of the outstanding questions regarding these
anomalous like-charge attractions.

Unlike atoms, which travel ballistically within the potential energy landscape established by inter-atomic interactions, colloidal particles are immersed in a viscous fluid that randomizes their trajectories over intervals longer than , the momentum relaxation time. Given a typical colloidal diffusion coefficient , . Consequently colloidal suspensions' microscopic temperatures are not easily monitored with the usual kinetic definition of the temperature, Eq. (2). The configurational temperature, by contrast, can be measured from snapshots and so provides an ideal alternative.

The fluid also acts as an intimately coupled heat bath whose heat capacity vastly exceeds the colloidal particles'. Consequently, the dispersion's thermodynamic temperature is all but guaranteed to be the fluid's, which is readily monitored with standard techniques. It is natural, therefore, to compare estimates of the configurational temperature based on microscopic dynamical measurements with this bulk thermodynamic temperature.

Instead, the particles' diffusion is often interpreted though the Einstein-Smoluchowsky relation to provide information on the viscous drag coefficient given knowledge of the systems' macroscopic thermodynamic temperature. The configurational temperature formalism offers alternatives for assessing the thermodynamic state of Brownian systems once the constituent particles' positions and pair interactions are known. Conversely, it offers additional tools for probing interactions for a system whose temperature can be established by other means.

Our samples consist of negatively charged silica spheres in diameter (Duke Scientific Lot. 24169) dispersed in water and confined within a slit pore of height formed between a glass microscope slide and a cover slip. The glass surfaces also develop large negative charge densities in contact with water (9), which repel the spheres and prevent them from sticking under the influence of van der Waals attraction. Silica spheres are roughly twice as dense as water and sediment to a height of roughly 300 nm above the lower wall in a matter of seconds (10). The low-concentration samples used in this study thus form a dilute monolayer once they reach equilibrium. Reservoirs of mixed-bed ion exchange resin help to maintain a total ionic strength around in the visible sample area.

The hermetically sealed sample is allowed to equilibrate at ambient temperatures on the stage of a microscope, with the bulk temperature being gauged with a standard mercury thermometer to an accuracy of about 0.5C. The particles' motions are imaged with a CCD (charge-coupled device) camera and video taped at 30 frames/sec before being digitized. Standard methods of digital video analysis (11) identify the particles in each video frame and report their locations in the plane with a resolution of 30 nm. The resulting distribution,

(24) |

of particles in the field of view at time provides detailed information regarding the particles' dynamics under the combined influences of random thermal forces and their mutual interactions. Distilling this information into an easily interpreted form requires further analysis.

One of the most commonly used tools for analyzing colloidal microscopy data is the radial distribution function, , which is computed as

(25) |

where is the areal density of particles in a field of view containing particles. Angle brackets in Eq. (25) denote averages over time, over angles and over the field of view, . A typical example appears in the inset to Fig. 1.

## § V.2. Colloidal interaction measurements

For a system with radially symmetric pairwise-additive interactions, the radial distribution function can be related to the pair potential, . As a starting point, we introduce the potential of mean force,

(26) |

which reduces to the pair potential in the dilute limit: . Crowding at higher concentrations induces many-body correlations that appear as oscillations both in and also in . Such oscillations arise even in systems with monotonically repulsive interactions, and could be mistaken for structure in . Reducing the concentration to avoid many-body correlations often is not practical given the competing requirements of tracking particles accurately (which favors high magnifications (12)) and amassing adequate statistics in (which favors a wide field of view and thus low magnification (10)).

No exact closed-form relationship between and is known for systems at finite concentrations. Fortunately, Henderson's uniqueness theorem (13) guarantees that any trial potential that reproduces uniquely describes the system's interactions, provided these are indeed pairwise additive. In light of this, two complementary methods for inverting to obtain have been introduced. The inverse Brownian dynamics (14) and inverse Monte Carlo (15) techniques compare the experimental radial distribution function with results obtained from numerical simulations based on experimental conditions and trial potentials until a match is obtained. The liquid structure inversion technique (16) uses the Ornstein-Zernike (OZ) integral equation (17) to correct for many-body correlations directly. This is the approach we will adopt.

The OZ equation describes the evolution of many-body correlations from a hierarchy of pairwise interactions. Truncating the hierarchy yields approximate but analytically tractable relationships between and . Among these, the hypernetted chain (HNC) approximation is found to be accurate for “soft” potentials while the Percus-Yevick (PY) approximation is more accurate for short-ranged interactions. For two-dimensional systems, the pair potential can be evaluated in these approximations as (18)

(27) |

where the convolution integral,

(28) |

is solved iteratively, starting with .

Provided the particles' concentration is not too high, and that their interactions are pairwise additive and neither too long-ranged nor too short, and that is known with sufficient accuracy, both approximations should yield equivalent results for . Unfortunately, consistency does not guarantee accuracy (10), and additional checks are necessary to ensure that pair potentials obtained with Eqs. (27) and (28) are meaningful. This is all the more important if such measurements as the example in Fig. 1, run counter to long-established theory (19). In this particular case, the deep minimum at is inconsistent with the purely repulsive interaction described by Eq. (23). Such anomalous interactions have been reported before in confined colloidal monoalyers (14); (20); (16); (19), but their origin has remained unresolved for more than a decade. Is this minimum a real albeit unexplained feature of the charged colloids' pair interaction, or is it an artifact of the measurement technique?

## § V.3. Temperature-based consistency tests for

Because hyperconfigurational temperatures depend sensitively on particles' interactions, they constitute a hierarchy of thermodynamic self-consistency tests for . The first successful application of the configurational temperature formalism to colloidal interaction measurements was reported in Ref. (5). Here, we provide a more detailed description of these tests' implementation and compare their results with those from thermodynamic sum rules. Together, this suite of independent tests confirms that pair potentials extracted from digital video analysis of confined charge-stabilized colloidal monolayers can be both thermodynamically self-consistent and accurate.

The flow chart in Fig. 2 outlines our strategy for checking and optimizing pair potentials. Given a candidate pair potential, we calculate a variety of configurational temperatures and compare these with the known bulk temperature at which the experiment was performed. Equation (27) conveniently yields in units of , so that configurational temperatures derived from are automatically normalized by the bulk thermodynamic temperature, . The condition for thermodynamic consistency therefore reduces to in these units. Successful collapse of the hierarchy of configurational temperatures to the thermodynamic temperature suggests that all of the conditions assumed in the configurational temperatures' derivations have been met and that accurately describes pairwise additive contributions to the free energy of a homogeneous isotropic system in equilibrium. Conversely, a failure to converge suggests that one or more of these conditions has not been met.

Identifying departures from equilibrium is particularly important in experimental systems where subtle fluid flows or environmental drifts can lead to correlations that might be mistaken for intrinsic interactions. Numerical simulations have demonstrated that the configurational temperature tracks sudden temperature jumps (2) far faster than the pressure or internal energy, and that its fluctuations also decay more rapidly, particularly for shorter-ranged potentials (21). These observations suggest that the configurational temperature may converge to a well-defined value even before the system achieves equilibrium. This surprising insensitivity has been ascribed (21) to the dominant contribution to the configurational temperature of forces among nearby pairs of particles, which can relax to near-equilibrium configurations long before a disturbance can propagate through the entire system. The configurational temperature, therefore, is a better probe of local equilibrium than global.

## § V.4. A sum rule for interaction measurements

Thermodynamic sum rules provide the necessary tests for global equilibrium. A particularly convenient form may be derived from Eq. (7) if the pair potential is radially symmetric. In this case,

(29) |

and we can explicitly calculate the thermodynamic averages of both sides of this equation:

(30) |

and

(31) |

Combining these results yields the sum rule

(32) |

This sum rule should apply at arbitrary areal densities for any system whose interactions can be described by a pairwise-additive central potential, . A similar result was obtained in Ref. (22) for three-dimensional systems.

Using the radial distribution function to average over pairs of particles removes any sensitivity to local structural variations, and thus focuses attention on global properties such as the degree of equilibration. Consequently, Eq. (32) complements the hierarchy of configurational temperature consistency checks.

## § V.5. Practical considerations

### § V.5.1. The range of interactions

These thermodynamic tests turn out to be exceedingly sensitive to imperfections in experimental data, and care is required to apply them meaningfully. For example, video microscopy data necessarily is restricted to a limited field of view, even if the sample itself is substantially larger. Particles near the edge of the field of view may have strongly interacting neighbors just out of sight whose contributions to their net force would be overlooked. The large apparently unbalanced forces due to this pernicious edge effect would grossly distort estimates of the configurational temperature were they included in averages such as Eq. (6).

To avoid this, we calculate force distributions only for particles whose relevant neighbors all lie within the field of view. Such particles lie no closer than the interaction's range to the edge of the field of view. We estimate from and by computing

(33) |

which heuristically describes an effective contribution to the configurational temperature of pairs of spheres interacting with potential at range (5). A typical example appears in Fig. 3. This should be considered no more than a heuristic guide because it neglects three-particle correlations, which are known (21) to be important for estimating the temperature.

Pairs at large enough separation that their interactions are vanishingly weak contribute negligibly to . The interaction range therefore can be estimated from the trailing edge of in Fig. 3. By considering particles no closer than to the edge of the field of view, we can ensure that no relevant pair interactions will be missed in calculating the full configurational temperature. The restricted field of view is indicated by the small rectangle overlaid on the photograph in Fig. 2. Given this cut on the data, we can proceed to calculate the full configurational temperature.

The data in Fig. 1 display two features whose validity we can assess using the configurational temperature formalism. The first is the anomalous minimum that can be interpreted as evidence for long-ranged attractions between like-charged particles. The second is the surprisingly weak contact repulsion. This low barrier to aggregation probably is not real, otherwise particles would aggregate rapidly by van der Waals attraction. Could the minimum similarly be an artifact?

Closer inspection of the data in Fig. 1
reveals that is finite even at , which should be impossible.
Two sources of experimental error, projection error due to particles' small
out-of-plane motions and polydispersity in the spheres' diameters,
artificially increase near contact and thus dramatically reduce
the apparent interaction energy near .
What Fig. 1 shows is the *effective potential*
consistent with the raw data for the particles' positions ,
including both of these contributions.
The configurational temperature calculation also is based on the same
raw position data and so requires
the associated *effective potential* as an input.
The consistency condition thus tests the
accuracy and thermodynamic self-consistency of for the measured
set of data.

Given and , the net force on the -th particle at time can be estimated using Eq. (5), with the sum over neighboring particles being restricted to those with . The set of single-particle forces then can be compiled into estimates for the configurational temperature for that particular snapshot, and a sequence of snapshots averaged to obtain a final result. Averaging is not necessary if each snapshot captures a large enough number of particles. The dilute samples in our study, however, typically yield so that several thousand frames are required for adequate statistics.

### § V.5.2. Influence of measurement errors

Even when care is taken to avoid edge effects, naively calculating forces, and thus temperatures, with the experimentally sampled yields unsatisfactory results, as the data in Fig. 4 demonstrate. This shows the histogram of values obtained from the 11912 video frames used to generate Fig. 1. Although the distribution is indeed peaked around unity, the average, , deviates wildly from the expected value. Other definitions also fare badly, with , for example. These extraordinarily large averages are not representative of the force distribution, however. Rather, they can be ascribed to a small number of frames with huge apparent temperatures. More disturbingly, many frames appear to have negative temperatures.

Frames with negative temperature must have . The inset to Fig. 4 shows that this is indeed the case. Such negative values should be extraordinarily rare in an equilibrated system because they signal mechanical instability. In this case, however, they can be ascribed to scatter in the experimentally determined potential, , which is greatly emphasized by the Laplacian operator. Particularly for where is close to 0, the signal-to-noise ratio becomes small and errors in the computed configurational temperature becomes unacceptably large. Cutting the long-range part of helps to some degree by substantially decreasing the number of frames with negative temperature. However, this does not suppress the high-temperature tail in the histogram of single-frame temperatures, as can be seen from the dashed plot in Fig. 5. These artifacts still boost the apparent temperature to an unrepresentative .

To minimize the influence of scatter in , we compute the configurational temperature using a fifth-order polynomial fit shown in Fig. 1 as the input potential. This simple smoothing procedure eliminates both negative and unreasonably large values of the calculated configurational temperature. The solid curves plotted in Fig. 5. show the resulting distribution of configurational temperatures factored along the orthogonal and directions in the field of view according to Eq. (10). The two component, and differ by less than 2.5%, which helps to confirm the system's isotropy. Their mean, , also is reassuringly close to the expected value.

Even when the temperature is calculated with the smoothed pair potential, a few frames still yield negative values for the configurational temperature. This is reasonable if and the pair potential has an extremum at an intermediate separation, . In this case must change sign and some particles may contribute negative values to the average in a snapshot. Chances improve for the average itself to be negative if is small. For example, 10 out of 11912 frames have negative apparent temperatures in a sample with . Individual snapshots can yield negative values, at least in principle, even for systems characterized by purely repulsive interactions, such as the example in Sec. V.5.6. In practice, however, none were observed.

### § V.5.3. Finite size scaling

The relatively small number of particles in the field of view has other ramifications. Because the various configurational temperature definitions involve different approximations of order , we might expect their results to differ from each other and from the actual thermodynamic temperature accordingly. Imaging a substantially larger region of the sample is not feasible. On the other hand, deliberately sub-sampling the field of view allows us to probe the dependence on sample size, for which we can extrapolate the configurational temperatures to the thermodynamic limit.

The data in Fig. 6 were obtained by calculating the configurational temperature with the field of view reduced by borders of 20, 40, 60, 80, 100, 120, 140, 160 and 180 pixels. The interaction range in this system is . Reducing the number of particles in this manner substantially changes the estimated temperatures, thereby confirming the importance of finite-size scaling. We fit these results to polynomials in with statistical weighting estimated from the area and the interaction range. These weighted fits describe the data very well, so that the extrapolation to should yield meaningful estimates for the configurational temperatures in the thermodynamic limit. Indeed, the extrapolated results, , and , agree quite well with each other, and all appear to be consistent with unity. Confirming thermodynamic consistency, however, requires us to assess the configurational temperatures' sensitivity to errors in .

### § V.5.4. Sensitivity to input potential

The apparently good extrapolation of the configurational temperatures to the thermodynamic temperature would offer little insight into the nature of the confined colloids' interactions if these results were insensitive to relevant features in the pair potential. For example, considerable attention has been paid in the literature to the possibility that anomalous confinement-induced like-charge attractions such as the example in Fig. 1 might be artifactual. However, if we truncate the negative region of to create a purely repulsive potential and recalculate the configurational temperatures, , and all extrapolate to 1.5, an error of . The large deviation resulting from this admittedly crude test suggests that the observed attraction is indeed an integral part of the charged particles' interaction.

More sensitive tests for thermodynamic consistency are provided by the hyperconfigurational temperatures defined in Eq. (9). Figure 7 demonstrates how small variations in can cause the hyperconfigurational temperatures to deviate with respect to each other and also with respect to the thermodynamic temperature. Two smoothed versions of the potential are plotted in Fig. 7(a), one fit over the range and the other over the more restricted . The former collapses the entire hierarchy of hyperconfigurational temperatures plotted in Fig. 7(b) to in the extrapolated thermodynamic limit, with , which compares favorably to in Fig. 6. The latter yields the far less satisfactory results in Fig. 7(c). Rather than collapsing onto the thermodynamic temperature, deviates systematically to lower values with larger index .

This qualitative difference is due to substantial contributions
from pairs of particles with .
Such pairs should not be present in a monodisperse sample of impenetrable
spheres, but appear in practice because of the sample's 3%
polydispersity in radius and because of projection errors due to the
particles' out-of-plane fluctuations.
These two effects are responsible for the observed correlations
at in Fig. 1, and for the unreasonably
small values of in the unphysical range .
The successful collapse of the configurational and
hyperconfigurational temperatures under these conditions
demonstrates that the
*effective* potential accounts
for the apparent particle distribution
and may differ subtly from the *true* pair potential.

The results in Fig. 7(b) and 7(c) reflect the general trend that higher order hyperconfigurational temperatures are more sensitive to details of the input potential. Even so, we can adjust the input potential within the experimental error bounds so that all of the hyperconfigurational temperatures converge to unity. In this sense, the hyperconfigurational temperatures not only strengthen our conclusions regarding the nature of anomalous like-charge attractions, but also enable us to improve our estimates for by adjusting for improved thermodynamic self-consistency.

The data in Fig. 7(c) also highlight another general feature of the configurational temperatures. Even though the more restricted trial potential does not successfully collapse the data, it does yield consistent results for configurational temperatures factored along orthogonal directions. This is a good indication that, indeed, the system is isotropic.

### § V.5.5. Checking for isotropy

Comparing temperatures measured along orthogonal directions not only provides a useful check for the interactions' isotropy, but also can be used to appraise the imaging system. For an isotropic sample, we expect that

(34) |

will vanish for *arbitrary* choices of .
More usually, the combination of a commercial video camera and
video frame grabber results in slightly different length scale
calibrations.
Even quite small differences measurably affect the temperatures calculated
from the associated force components if the interaction is assumed
to be isotropic.
For example, we found that
for the confined sample at
and for the sample at .
Even such small differences are plainly visible in
Fig. 7(c).

Apparent anisotropies of this magnitude appear consistently in our data sets regardless of the samples' composition, concentration, degree of confinement, and so are unlikely to reflect statistical errors. Nor are they likely to signal a real anisotropy in our samples' interactions. Instead, they result from the hyperconfigurational temperatures' sensitivity to subtle geometric distortion in our imaging system. Rescaling the measured and coordinates slightly can substantially reduce the apparent anisotropy in the entire hierarchy of hyperconfigurational temperatures, as the data in Table 1 show. For the system used in this study, a 0.7% correction of the scale ratio is enough to account for the 5.8% anisotropy of in the data.

The same scaling factor also corrects the apparent anisotropy in the other samples we have studied, and thus appears to be correctly interpreted as a correction to the calibration of our imaging system. Furthermore, differences in the scaling factors as small as % perform substantially less well, as shown by the data in Table 1. This level of sensitivity greatly exceeds the typical 1% calibration accuracy obtained by imaging test patterns, and thus provides a new tool for assessing and correcting geometric defects in the digital video microscopy system.

scaling factor | |||||

1:1 | 1.003:0.997 | 1.003:0.9963 | 1.003:0.996 | ||

1 | 5.8% | 0.61% | -0.07% | -0.82% | |

3 | 4.4% | 0.87% | 0.009% | -0.20% | |

5 | 2.6% | 1.3% | 0.16% | 0.90% | |

7 | 1.4% | 1.8% | 0.65% | 0.71% |

Successfully correcting apparent anisotropy in the hyperconfigurational temperatures also provides insight into the nature of the system's interactions. If replacing by an arbitrary function causes to differ from then the system indeed may be anisotropic, either because its interactions are anisotropic, or else because of its response to an external field. In the latter case, the external field contributes an additional configuration-dependent term to the Hamiltonian, , which contributes, in turn, to the definitions of the configurational temperatures. If , then the configurational temperatures along the two directions generally will differ.

### § V.5.6. Accounting for sample imperfections in

Colloidal samples quite often include small populations of aggregated pairs of spheres, also known as dimers. These can have a striking effect on the configurational temperature, as the data in Fig. 8 demonstrate. This sample also consists of diameter silica spheres, but sedimented to the bottom wall of a slit pore thick at an areal density . Unlike samples in relatively thin () slit pores considered so far, such weakly confined silica monolayers are found to have purely repulsive screened-Coulomb interactions (10); (19) in at least qualitative agreement with mean-field theory. The presence of a few dimers, however, contribute a small peak to the radial distribution function at whose tail extends to . These excess correlations are dramatically magnified in because any two nominally repulsive particles ought to exert exceptionally large forces on each other near contact. The overall result is extremely large configurational temperatures.

At first glance, such defects in the sample would appear to render meaningful measurements of the configurational temperature impossible. However, the function can be used to cut the spurious data while retaining enough useful information for an accurate assessment. In particular, the peak in at small is well separated from the principal peak at . This suggests that the former can be ascribed entirely to dimers and the latter to genuine long-ranged interactions, with a clean division at about . It seems reasonable, therefore, to eliminate dimers' contribution to the configurational temperatures by excluding any pair separations smaller than .

Restricting the range of too much would exclude relevant interactions, causing the net force on many particles to appear unbalanced and the configurational temperature to increase accordingly. For the present data set, we find that increasing the lower cutoff to only increases the apparent temperature by a few percent. This indicates that three-body correlations are weak at this concentration range and that the proposed cutoff at will not distort the results. Similarly, ignoring contributions from pairs separated by more than has little influence on the estimated temperature and establishes the interaction range for accurate temperature estimates.

Figure 9 shows typical finite-size scaling results for a trial fourth-order polynomial fit to the experimentally obtained pair potential over the range . The fit potential is plotted as a dashed curve in Fig. 10(a). Replacing this with a fit to the predicted mean field potential described by Eq. (23) over the range yields comparably good convergence, and increasing the range to decreases the extrapolated configurational temperature by just 4 %. All of these trial potentials fall within error estimates for the measured potential. On this basis, and because the various definitions of the configurational temperature all extrapolate to unity, we conclude that the measured potential once again accurately describes the system's equilibrium pair interactions. In this case, however, the potential appears to be purely repulsive.

The sample used to compile these data also included one particle that had deposited irreversibly onto the lower glass surface near the edge of the field of view. A single immobilized sphere might not be expected to influence the free monolayer's structure and dynamics much. The influence on the radial distribution function is indeed subtle, with the slight peak at in (Fig. 8) disappearing when the region containing the stuck particle is excluded from the calculation. The effect on the candidate pair potential is somewhat more pronounced, particularly for , as can be seen in Fig. 10(a). But does the potential from the restricted data set better reflect the system's interactions? After all, the configurational temperatures calculated with the unrestricted data in Fig. 9 all extrapolate reasonably well to the thermodynamic value. Figures 10(b) and 10(c) show that the hierarchy of hyperconfigurational temperatures collapses to unity only when the region containing the stuck particle is excluded. The restricted data set, therefore, offers a more accurate picture of the pair potential. This is consistent with our earlier observation that small uncertainties in at small separations can be dramatically magnified in .

## § V.6. Applying the thermodynamic sum rule

Even when the configurational and hyperconfigurational temperatures converge to the thermodynamic value for a reasonable choice of pair potential, the system's degree of equilibration still has to be assessed. Figure 11 shows the integrand of the sum rule in Eq. (32) for two slightly different effective potentials, both of which are consistent with the interactions measured in the confined colloidal monolayer at . Even differences in the input potential too small to affect the configurational temperatures can change the sum rule's integrand substantially. These changes affect whether or not the sum rule as a whole is satisfied.

Normalizing the integral in Eq. (32) by the integral of the integrand's absolute value provides a useful measure of convergence. The best-fit pair potential obtained from the liquid structure inversion (plotted as circles in the inset to Fig. 11) yields an unacceptably large relative error of 0.5. At such low areal densities (), is very similar to the potential of mean force, , and very small changes in can influence the sum rule's integrand substantially. In fact, adjusting the potential just slightly to the function plotted as squares in Fig. 11 improves the sum rule's convergence to 0.001. This modified potential still successfully collapses the configurational and hyperconfigurational temperatures, and thus may be considered an improved estimate for the pair potential. This successful application of the thermodynamic sum rule suggests that the system is indeed in equilibrium, and that the candidate pair potential, including its long-ranged attraction, accurately describes the confined particles' interactions.

## § V.7. Areal pressures

The foregoing considerations serve to confirm that the same colloidal silica spheres experience qualitatively different equilibrium pair interactions when confined between parallel glass walls separated by and , with the more strongly confined dispersion exhibiting anomalous long-ranged attractions. Such a qualitative difference in the system's pair potential also should manifest itself in the system's other thermodynamic properties, such as its pressure.

Given the particles' locations and an estimate for their pair interactions, we can calculate the monolayers' pressure as , where is a departure from ideal gas behavior due to the particles' interactions. This virial pressure is given by

(35) |

where is the dimension of the space, and . The former definition based on absolute coordinates works best for systems with open boundaries, which are described in the grand canonical ensemble, but fails in systems with periodic boundary conditions, which often are used in computer simulations. The latter definition based on relative coordinate works in both. Either should apply to our experimental data.

Comparing the different systems' interaction-driven departures from ideal gas behavior is facilitated by defining

(36) |

whose dependence on system size is plotted in Fig. 12. These data were obtained with the definition based on absolute coordinates measured from the center of the field of view. Equivalent results obtained with relative coordinates differ by less than 1.5%. As for the configurational temperature definitions, Eq. (35) applies in the thermodynamic limit, and is obtained by extrapolating to large system size. In this case, we see that the system at with long-ranged repulsive interactions has a substantially higher pressure than would be expected for an ideal gas at the same areal density. The increase is much smaller in the more strongly confined system, presumably because of the potential's long-ranged attractive tail.

Like the configurational temperature, the pressure also depends sensitively on the input potential and length scale calibration. Without appropriate rescaling, is 10% higher than in the data set. After rescaling using the factors obtained by requiring isotropy in the configurational temperatures, the two components agree well, as can be seen in Fig. 12. This further confirms that the observed anisotropy is due to artificial image distortion that can be corrected with a single rescaling factor.

## § V.8. Using to measure

(nm) | |||||
---|---|---|---|---|---|

160 | 10147 | ||||

180 | 6755 | ||||

200 | 4779 | ||||

210 | 4096 | ||||

220 | 3549 | ||||

250 | 2438 |

The configurational and hyperconfigurational temperatures
show great promise as thermodynamic self-consistency tests
for interaction measurements in soft-matter system.
They also can be used to measure interactions directly, with
the hierarchy of definitions providing a set of simultaneous
constraints on free parameters in a model for the pair potential.
Furthermore, even when the theoretical form of the potential is
not known *a priori*, we believe that a numerically determined function
that simultaneously collapses the entire hierarchy to the thermodynamic
temperature would provide a model-free estimate for the potential.
For example, we might approximate the pair potential with a polynomial
whose coefficients would be fixed by numerically seeking best collapse
of the data.

As an example of this procedure, we use hyperconfigurational temperatures to determine the parameters and in the screened-Coulomb potential, Eq. (23), that accounts for the distribution of particles in the data set (19). Requiring yields a screening length of and an effective charge on the particles of . These values also converge and close to unity, suggesting that the screened-Coulomb potential reasonably describes the interactions in this system. Table 2 shows typical results for parameters near the optimal values.

Simply fitting Eq. (23) to the measured pair potential in Fig. 10(a) yields and . Although these values differ somewhat from those obtained by collapsing the configurational temperatures, both are broadly consistent with the charge regulation theory for interacting silica surfaces (9). Their disagreement and the slight departure from unity of the highest-order hyperconfigurational temperatures suggests a small deviation of the true pair potential from the predicted screened-Coulomb form. This is reasonable because the nearby bottom wall probably affects the interaction somewhat. No convergence is possible at all for the data at because the pair interactions are not well described by the purely repulsive screened-Coulomb form.

Using hyperconfigurational temperatures to provide constraints on models for the pair potential offers advantages over other interaction measurement techniques. Principally, this method eliminates the intermediate steps of first measuring and then inverting it to obtain . This first step generally requires amassing large amounts of data to obtain accurate information on correlations at small separations. The second involves uncontrolled approximations and limits the range of concentrations over which interactions can be measured. By contrast, the present approach requires substantially less data and involves fewer approximations. Consequently, this approach should be useful for exploring denser, more strongly interacting systems. Its ability to distinguish pairwise and many-body interactions also should be helpful for such studies.

On the other hand, calculating configurational temperatures involves sums over all pairs of particles, and must be repeated for sub-samples of varying size to account for finite-size scaling. This can be computationally expensive, particularly in model-free searches. Implementing fast search algorithms on restricted data sets with additional data being added only in the final stages of polishing should alleviate this problem.

Failure of such a search to converge may signal a failure of pairwise additivity, a departure from equilibrium, or else an inappropriate model for the pair potential. Distinguishing these would probably require resorting to one of the alternate measurement methods.