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The preceding sections describe image analysis methods we have developed to perform quantitative time-resolved imaging studies of colloidal suspensions. This presentation emphasized techniques which offer high accuracy while requiring only general purpose commercially available equipment. The demonstrated spatial resolution of 10 nm in the plane and 150 nm in depth for tex2html_wrap_inline1193 m radius spheres should suffice for a wide range of applications. The two applications we discussed in detail, measurement of microspheres' self-diffusion coefficients and measurement of charged spheres' pair interaction potential, demonstrate the utility of these methods in studying the microscopic dynamics of colloidal suspensions. This work was supported by the National Science Foundation under Grant No. DMR-9320378.

David G. Grier
Mon Mar 11 23:01:27 CST 1996