Holographic Video Microscopy
 
The setup
 
  1.         Our in-line holographic video microscope shown is based on an inverted microscope  outfitted with a 100x numerical aperture 1.4 oil immersion objective.
 
  1.         The conventional incandescent illumination is replaced with the collimated coherent beam from either a He-Ne laser or a solid-state laser .
 
  1.         Individual colloidal spheres scatter a small proportion of the incident beam, and the scattered light interferes with the unscattered portion in the focal plane of the microscope's objective lens.
 
  1.         The microscope magnifies the interference pattern and projects it onto the face of a low-noise gray-scale video camera (NEC TI 324 IIA), with a total system magnification of 101 nm/pixel.
  2.         The video stream is recorded as uncompressed digital video with a digital video recorder (Pioneer H520S) for subsequent analysis.
 
The Summary of the analysis method
 
  1. 1.        Analyze a video DVD with Transcode to obtain individual frames/ use any frame grabbing software to do split the video into individual frame.
  2. 2.        Download and install all the necessary IDL codes from the software page.
  3. 3.        Estimate the starting point of the fit (spheretool.pro)
  4. 4.        Loop the tracking procedure for all frames
  5. 5.        Link the coordinates found in each frame together to form trajectories.
  6. 6.        Analyze the trajectory info as you see fit for your particular experiment.
 
    •    Click here for a tutorial (updated 01-09-09)
    •    Click here to download the IDL code for HVM (updated 01-09-09)
 
 
 
HVM standard Setup