The preceding sections describe image analysis methods we have developed
to perform quantitative time-resolved imaging studies of colloidal suspensions.
This presentation emphasized techniques which offer high accuracy
while requiring only general purpose commercially available equipment.
The demonstrated spatial resolution of 10 nm in the plane and 150 nm
in depth for
m radius spheres should suffice for a wide
range of applications.
The two applications we discussed in detail, measurement of
microspheres' self-diffusion coefficients and measurement of
charged spheres' pair interaction potential, demonstrate the utility
of these methods in studying the microscopic dynamics of colloidal
suspensions.
This work was supported by the National Science Foundation under
Grant No. DMR-9320378.